Article
Pilot study to determine the feasibility of producing protease-resistant prion protein fragments by random PCR mutagenesis.
Biochemical and biophysical research communications - 9 Mar 2001
Dear D V, Fitzmaurice T J, Garton S, Richards S J
Abstract excerpt
We report the results of a pilot study to determine the feasibility of using PCR random mutagenesis and in vitro transcription/translation to produce protease resistant full-length or truncated ovine prion proteins (PrP). Using this approach, we show the novel production of protease resistant recombinant ovine prion protein fragments isolated from a panel of seventy randomly mutated ovine PrP protein molecules....
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