Article
A cautionary note on the use of stable transformed cells.
Apoptosis : an international journal on programmed cell death - 1 Apr 2000
Blum D, Torch S, Nissou M F, Verna J M
Abstract excerpt
Gene transfection and ectopic expression is a widely used method in experimental biology. In the present report, we would like to point out that this approach may, in certain circumstances, lead to a modification of the transfected cell phenotype. Indeed, we observed that after transfection of bcl-2 gene in the neuronal PC12 cell line some of the selected clones have lost their neuronal and catecholaminergic...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.