Article
Endocytic sorting of CFTR variants monitored by single-cell fluorescence ratiometric image analysis (FRIA) in living cells.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Barrière Herve, Apaja Pirjo, Okiyoneda Tsukasa, Lukacs Gergely L
Abstract excerpt
The wild-type CFTR channel undergoes constitutive internalization and recycling at the plasma membrane. This process is initiated by the recognition of the Tyr- and di-Leu-based endocytic motifs of CFTR by the AP-2 adaptor complex, leading to the formation of clathrin-coated vesicles and the channel delivery to sorting/recycling endosomes. Accumulating evidence suggests that conformationally defective mutant...
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