Article
Three-color flow cytometry analysis of tricistronic expression of eBFP, eGFP, and eYFP using EMCV-IRES linkages.
Cytometry - 1 Sept 1999
Zhu J, Musco M L, Grace M J
Abstract excerpt
BACKGROUND: The ability to quickly analyze and sort double or triple fluorescent reporter constructs using simultaneous analysis provides significant flexibility in the solution of analytical and process-related questions in biotechnology. METHODS: Bicistronic eBFP/eGFP and eBFP/eYFP constructs were made on two mammalian episomal plasmids using an internal ribosomal entry sequence from encephalomyocarditis virus...
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