Article
A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy.
Nature biotechnology - 1 May 2006
Kogure Takako, Karasawa Satoshi, Araki Toshio, Saito Kenta, Kinjo Masataka, Miyawaki Atsushi
Abstract excerpt
Dual-color fluorescence cross-correlation spectroscopy (FCCS) is a promising technique for quantifying protein-protein interactions. In this technique, two different fluorescent labels are excited and detected simultaneously within a common measurement volume. Difficulties in aligning two laser lines and emission crossover between the two fluorophores, however, make this technique complex. To overcome these...
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