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Visualizing levels of osteoblast differentiation by a two‐color promoter‐GFP strategy: Type I collagen‐GFPcyan and osteocalcin‐GFPtpz

7 Sept 2005

Abstract excerpt

A 3.9 kb DNA fragment of human osteocalcin promoter and 3.6 kb DNA fragment of the rat collagen type1a1 promoter linked with visually distinguishable GFP isomers, topaz and cyan, were used for multiplex analysis of osteoblast lineage progression. Three patterns of dual transgene expression can be appreciated in primary bone cell cultures derived from the transgenic mice and by histology of their corresponding...

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PMID: 16149065
DOI: 10.1002/gene.20156

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