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Article

Site-directed mutagenesis of K396R of the 65 kDa glutamic acid decarboxylase active site obliterates enzyme activity but not antibody binding.

FEBS letters - 19 Jan 2001

Hampe C S, Hammerle L P, Falorni A, Robertson J, Lernmark A

Abstract excerpt

The role of K396 in the enzymatic catalysis and the antigenicity of the 65 kDa isoform of glutamate decarboxylase (GAD65) was analyzed using the K396R GAD65 mutant. GAD65 is a major autoantigen in Type 1 diabetes and autoantibodies directed to GAD65 are widely used markers for this disease. We found that (1) recombinant human GAD65 is fully enzymatically active; (2) the K396R mutation abolished GAD65 activity;...

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PMID: 11163769
DOI: 10.1016/s0014-5793(00)02429-7

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