Article
Conformation-dependent GAD65 autoantibodies in diabetes.
Diabetologia - 1 Sept 2004
Luo D, Gilliam L K, Greenbaum C, Bekris L, Hampe C S, Daniels T, Richter W, Marcovina S M, Rolandsson O, Landin-Olsson M, Kockum I, Lernmark A
Abstract excerpt
AIMS/HYPOTHESIS: Conformation-dependent autoantibodies directed against GAD65 are markers of Type 1 diabetes. In this study we aimed to determine whether the substitution of GAD65 with GAD67 amino acids would affect the binding of conformation-dependent GAD65 autoantibodies. METHODS: We used PCR-based site-directed mutagenesis to generate a series of mutated GAD65 cDNA constructs in which specific GAD65 coding...
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