Article
Recombinant human GAD65 accumulates to high levels in transgenic tobacco plants when expressed as an enzymatically inactive mutant.
Plant biotechnology journal - 1 Oct 2010
Avesani Linda, Vitale Alessandro, Pedrazzini Emanuela, Devirgilio Maddalena, Pompa Andrea, Barbante Alessandra, Gecchele Elisa, Dominici Paola, Morandini Francesca, Brozzetti Annalisa, Falorni Alberto, Pezzotti Mario
Abstract excerpt
The 65-kDa isoform of glutamic acid decarboxylase (GAD65) is the major autoantigen implicated in the development of type 1 diabetes mellitus (T1DM). The bulk manufacture of GAD65 is a potential issue in the fight against T1DM but current production platforms are expensive. We show that a catalytically inactive form of GAD65 (GAD65mut) accumulates at up to 2.2% total soluble protein in transgenic tobacco leaves,...
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