Article
Site-directed mutations in the transmembrane domain M3 of human connexin37 alter channel conductance and gating.
Biochemical and biophysical research communications - 19 Jan 2001
Kumari S S, Varadaraj K, Valiunas V, Brink P R
Abstract excerpt
Connexin37 (Cx37) is expressed principally in endothelial cells. We have introduced individual point mutations (Cx37-V156D or Cx37-K162E) in the putative pore lining segment M3 of a polymorphic human Cx37 (Cx37-S319) and expressed them in N2A and RIN cells. RT-PCR and immunofluorescence microscopy were used to confirm the expression of the proteins. Stably transfected cells were subjected to electrophysiological...
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