Article
Four new monomeric insulins obtained by alanine scanning the dimer-forming surface of the insulin molecule.
Protein engineering - 1 Nov 2000
Chen H, Shi M, Guo Z Y, Tang Y H, Qiao Z S, Liang Z H, Feng Y M
Abstract excerpt
The residues A21Asn, B12Val, B16Tyr, B24Phe, B25Phe, B26Tyr and B27Thr, buried in the dimer of insulin, were identified by means of alanine-scanning mutagenesis. The receptor binding activity, in vivo biological potency and self-association properties of the seven single alanine human insulin mutants were determined. Four of the seven single alanine mutants, [B12Ala]human insulin, [B16Ala]human insulin,...
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