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Article

Alanine scanning mutagenesis of insulin.

The Journal of biological chemistry - 16 May 1997

Kristensen C, Kjeldsen T, Wiberg F C, Schäffer L, Hach M, Havelund S, Bass J, Steiner D F, Andersen A S

Abstract excerpt

Alanine scanning mutagenesis has been used to identify specific side chains of insulin which strongly influence binding to the insulin receptor. A total of 21 new insulin analog constructs were made, and in addition 7 high pressure liquid chromatography-purified analogs were tested, covering alanine substitutions in positions B1, B2, B3, B4, B8, B9, B10, B11, B12, B13, B16, B17, B18, B20, B21, B22, B26, A4, A8,...

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Identifiers

PMID: 9148904
DOI: 10.1074/jbc.272.20.12978

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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