Article
Combined transformation and genetic technique verification of protein-protein interactions in the yeast two-hybrid system.
Biochemical and biophysical research communications - 2 Nov 2000
Tyagi S, Lal S K
Abstract excerpt
The yeast two-hybrid system is frequently used to identify protein-protein interactions. The assay is based on the functional reconstitution of a transcriptional activator. Since an indirect phenotype of the positive clones is the basis for selection of positive interacting clones, the two-hybrid...
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