Article
Homologous recombinational repair of double-strand breaks in yeast is enhanced by MAT heterozygosity through yKU-dependent and -independent mechanisms.
Genetics - 1 Feb 2001
Clikeman J A, Khalsa G J, Barton S L, Nickoloff J A
Abstract excerpt
DNA double-strand breaks (DSBs) are repaired by homologous recombination (HR) and nonhomologous end-joining (NHEJ). NHEJ in yeast chromosomes has been observed only when HR is blocked, as in rad52 mutants or in the absence of a homologous repair template. We detected yKu70p-dependent imprecise NHEJ at a frequency of approximately 0.1% in HR-competent Rad+ haploid cells. Interestingly, yku70 mutation increased...
Topics
- Alleles
- Antigens, Nuclear
- Cell Division
- DNA Damage
- DNA Helicases
- DNA Repair
- DNA-Binding Proteins
- Diploidy
- Haploidy
- Heterozygote
- Ku Autoantigen
- Mating Factor
- Models, Genetic
- Mutation
- Nuclear Proteins