Article
Role of two histidines in the (6-4) photolyase reaction.
The Journal of biological chemistry - 30 Mar 2001
Hitomi K, Nakamura H, Kim S T, Mizukoshi T, Ishikawa T, Iwai S, Todo T
Abstract excerpt
The reaction mechanism of Xenopus (6-4) photolyase was investigated using several mutant enzymes. In the active site, which is homologous between the cis,syn-cyclobutane pyrimidine dimer and (6-4) photolyases, four amino acid residues that are specific to (6-4) photolyase, Gln(288), His(354), Leu(355), and His(358), and two conserved tryptophans, Trp(291) and Trp(398), were substituted with alanine. Only the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.