Article
Identification of DNA cis elements essential for expansion of ribosomal DNA repeats in Saccharomyces cerevisiae.
Molecular and cellular biology - 1 Jan 2001
Kobayashi T, Nomura M, Horiuchi T
Abstract excerpt
Saccharomyces cerevisiae carries approximately 150 ribosomal DNA (rDNA) copies in tandem repeats. Each repeat consists of the 35S rRNA gene, the NTS1 spacer, the 5S rRNA gene, and the NTS2 spacer. The FOB1 gene was previously shown to be required for replication fork block (RFB) activity at the RFB site in NTS1, for recombination hot spot (HOT1) activity, and for rDNA repeat expansion and contraction. We have...
Topics
- DNA Replication
- DNA, Ribosomal
- DNA-Binding Proteins
- Electrophoresis, Agar Gel
- Electrophoresis, Gel, Two-Dimensional
- Fungal Proteins
- Gene Dosage
- Genes, Fungal
- Models, Genetic
- Mutation
- Plasmids
- Recombination, Genetic
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins