Article
Ribosomal DNA replication fork barrier and HOT1 recombination hot spot: shared sequences but independent activities.
Molecular and cellular biology - 1 Jul 2000
Ward T R, Hoang M L, Prusty R, Lau C K, Keil R L, Fangman W L, Brewer B J
Abstract excerpt
In the ribosomal DNA of Saccharomyces cerevisiae, sequences in the nontranscribed spacer 3' of the 35S ribosomal RNA gene are important to the polar arrest of replication forks at a site called the replication fork barrier (RFB) and also to the cis-acting, mitotic hyperrecombination site called HOT1. We have found that the RFB and HOT1 activity share some but not all of their essential sequences. Many of the...
Topics
- DNA Replication
- DNA, Fungal
- DNA, Ribosomal
- Deoxyribonuclease HindIII
- Deoxyribonucleases, Type II Site-Specific
- Mutation
- Recombination, Genetic
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transcription Factors