Article
Transgene integration into the same chromosome location can produce alleles that express at a predictable level, or alleles that are differentially silenced.
Genes & development - 15 Nov 2000
Day C D, Lee E, Kobayashi J, Holappa L D, Albert H, Ow D W
Abstract excerpt
In an effort to control the variability of transgene expression in plants, we used Cre-lox mediated recombination to insert a gus reporter gene precisely and reproducibly into different target loci. Each integrant line chosen for analysis harbors a single copy of the transgene at the designated target site. At any given target site, nearly half of the insertions give a full spatial pattern of transgene...
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