Article
In vitro selection of an RNA sequence that interacts with high affinity with thymidylate synthase.
Nucleic acids research - 1 Nov 2000
Lin X, Mizunuma N, Chen T, Copur S M, Maley G F, Liu J, Maley F, Chu E
Abstract excerpt
Previous studies have shown that the repressive effect of thymidylate synthase (TS) mRNA translation is mediated by direct binding of TS itself to two cis-acting elements on its cognate mRNA. To identify the optimal RNA nucleotides that interact with TS, we in vitro synthesized a completely degenerate, linear RNA pool of 25 nt and employed in vitro selection to isolate high affinity RNA ligands that bind human TS...
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