Article
Role of cysteine amino acid residues on the RNA binding activity of human thymidylate synthase.
Nucleic acids research - 15 Aug 2003
Lin Xiukun, Liu Jun, Maley Frank, Chu Edward
Abstract excerpt
The role of cysteine sulfhydryl residues on the RNA binding activity of human thymidylate synthase (TS) was investigated by mutating each cysteine residue on human TS to a corresponding alanine residue. Enzymatic activities of TS:C43A and TS:C210A mutant proteins were nearly identical to wild-type TS, while TS:C180A and TS:C199A mutants expressed >80% of wild-type enzyme activity. In contrast, TS:C195A was...
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