Article
Contributions of Torpedo nicotinic acetylcholine receptor gamma Trp-55 and delta Trp-57 to agonist and competitive antagonist function.
The Journal of biological chemistry - 26 Jan 2001
Xie Y, Cohen J B
Abstract excerpt
Results of affinity-labeling studies and mutational analyses provide evidence that the agonist binding sites of the nicotinic acetylcholine receptor (nAChR) are located at the alpha-gamma and alpha-delta subunit interfaces. For Torpedo nAChR, photoaffinity-labeling studies with the competitive antagonist d-[(3)H]tubocurarine (dTC) identified two tryptophans, gammaTrp-55 and deltaTrp-57, as the primary sites of...
Topics
- Acetylcholine
- Animals
- Binding Sites
- Dose-Response Relationship, Drug
- Ion Channel Gating
- Mutation
- Nicotinic Agonists
- Nicotinic Antagonists
- Photoaffinity Labels
- Protein Subunits
- Receptors, Nicotinic
- Recombinant Proteins
- Torpedo
- Tryptophan
- Tubocurarine