Mutations in the TATA-binding protein, affecting transcriptional activation, show synthetic lethality with the TAF145 gene lacking the TAF N-terminal domain in Saccharomyces cerevisiae.

doi:10.1074/jbc.M008208200

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Mutations in the TATA-binding protein, affecting transcriptional activation, show synthetic lethality with the TAF145 gene lacking the TAF N-terminal domain in Saccharomyces cerevisiae.

The Journal of biological chemistry - 5 Jan 2001

Kobayashi A, Miyake T, Ohyama Y, Kawaichi M, Kokubo T

Abstract excerpt

The general transcription factor TFIID, which is composed of the TATA box-binding protein (TBP) and a set of TBP-associated factors (TAFs), is crucial for both basal and regulated transcription by RNA polymerase II. The N-terminal small segment of yeast TAF145 (yTAF145) binds to TBP and thereby inhibits TBP function. To understand the physiological role of this inhibitory domain, which is designated as TAND (TAF...

Topics

Amino Acid Substitution
Cell Cycle Proteins
DNA-Binding Proteins
Fungal Proteins
Gene Expression Regulation, Fungal
Genes, Fungal
Genes, Lethal
Genes, Reporter
Macromolecular Substances
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phenotype

Precipitin Tests

Protein Structure, Tertiary

Protein Subunits

RNA Polymerase II

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

TATA-Binding Protein Associated Factors

Source

Open in PubMed

Identifiers

PMID: 11035037
DOI: 10.1074/jbc.M008208200

Genes

TBP
NSL1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutations in the TATA-binding protein, affecting transcriptional activation, show synthetic lethality with the TAF145 gene lacking the TAF N-terminal domain in Saccharomyces cerevisiae.PMID 11035037Read ↗

Mutations in the histone fold domain of the TAF12 gene show synthetic lethality with the TAF1 gene lacking the TAF N-terminal domain (TAND) by different mechanisms from those in the SPT15 gene encoding the TATA box-binding protein (TBP).Semantic proximity Read ↗

Impaired core promoter recognition caused by novel yeast TAF145 mutations can be restored by creating a canonical TATA element within the promoter region of the TUB2 gene.Semantic proximity Read ↗

Identification of highly conserved amino-terminal segments of dTAFII230 and yTAFII145 that are functionally interchangeable for inhibiting TBP-DNA interactions in vitro and in promoting yeast cell growth in vivo.Semantic proximity Read ↗

Structural and functional analysis of mutations along the crystallographic dimer interface of the yeast TATA binding protein.Semantic proximity Read ↗

TBP-associated factors are not generally required for transcriptional activation in yeast.Semantic proximity Read ↗

SPN1, a conserved gene identified by suppression of a postrecruitment-defective yeast TATA-binding protein mutant.Semantic proximity Read ↗

TFIID and Spt-Ada-Gcn5-acetyltransferase functions probed by genome-wide synthetic genetic array analysis using a Saccharomyces cerevisiae taf9-ts allele.Semantic proximity Read ↗

SPT3 interacts with TFIID to allow normal transcription in Saccharomyces cerevisiae.Semantic proximity Read ↗

SPT20/ADA5 encodes a novel protein functionally related to the TATA-binding protein and important for transcription in Saccharomyces cerevisiae.Semantic proximity Read ↗

Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly.Semantic proximity Read ↗

A TATA-binding protein mutant defective for TFIID complex formation in vivo.Semantic proximity Read ↗

Impaired uptake and/or utilization of leucine by Saccharomyces cerevisiae is suppressed by the SPT15-300 allele of the TATA-binding protein gene.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.