Article
The absence of ribonuclease H1 or H2 alters the sensitivity of Saccharomyces cerevisiae to hydroxyurea, caffeine and ethyl methanesulphonate: implications for roles of RNases H in DNA replication and repair.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Oct 2000
Arudchandran A, Cerritelli S, Narimatsu S, Itaya M, Shin D Y, Shimada Y, Crouch R J
Abstract excerpt
BACKGROUND: RNA of RNA-DNA hybrids can be degraded by ribonucleases H present in all organisms including the eukaryote Saccharomyces cerevisiae. Determination of the number and roles of the RNases H in eukaryotes is quite feasible in S. cerevisiae. RESULTS: Two S. cerevisiae RNases H, related to Escherichia coli RNase HI and HII, are not required for growth under normal conditions, yet, compared with wild-type...
Topics
- Caffeine
- Cell Cycle
- Cell Nucleus
- DNA Repair
- DNA Replication
- DNA, Fungal
- Ethyl Methanesulfonate
- Gene Deletion
- Genes, Fungal
- Hydroxyurea
- Nucleic Acid Synthesis Inhibitors
- Phenotype
- Recombinant Fusion Proteins