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Article

Excision of misincorporated ribonucleotides in DNA by RNase H (type 2) and FEN-1 in cell-free extracts.

Proceedings of the National Academy of Sciences of the United States of America - 24 Dec 2002

Rydberg Bjorn, Game John

Abstract excerpt

Misincorporated ribonucleotides in DNA will cause DNA backbone distortion and may be targeted by DNA repair enzymes. Using double-stranded oligonucleotide probes containing a single ribose, we demonstrate a robust activity in human, yeast, and Escherichia coli cell-free extracts that nicks 5' of the ribose. The human and yeast extracts also make a subsequent cut 3' of the ribonucleotide releasing a ribonucleotide...

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Identifiers

PMID: 12475934
DOI: 10.1073/pnas.262591699
PMCID: PMC139199

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