Article
Assignment of the contribution of the tryptophan residues to the spectroscopic and functional properties of the ribotoxin alpha-sarcin.
Proteins - 15 Nov 2000
de Antonio C, Martínez del Pozo A, Mancheño J M, Oñaderra M, Lacadena J, Martínez-Ruiz A, Pérez-Cañadillas J M, Bruix M, Gavilanes J G
Abstract excerpt
alpha-Sarcin, a potent cytotoxic protein from Aspergillus giganteus, contains two tryptophan residues at positions 4 and 51. Two single, W4F and W51F, and the double mutant, W4/51F, have been produced and purified to homogeneity. These two residues are neither required for the highly specific ribonucleolytic activity of the protein on the ribosomes (production of the so called alpha-fragment) nor for its...
Topics
- Anthracenes
- Circular Dichroism
- Cytotoxins
- Endoribonucleases
- Fluorescence Polarization
- Fungal Proteins
- Hot Temperature
- Models, Molecular
- Molecular Probes
- Mutation