Article
NMR structure of the noncytotoxic alpha-sarcin mutant Delta(7-22): the importance of the native conformation of peripheral loops for activity.
Protein science : a publication of the Protein Society - 1 Apr 2004
García-Mayoral Ma Flor, García-Ortega Lucia, Lillo Ma Pilar, Santoro Jorge, Martínez del Pozo Alvaro, Gavilanes José G, Rico Manuel, Bruix Marta
Abstract excerpt
The deletion mutant Delta(7-22) of alpha-sarcin, unlike its wild-type protein counterpart, lacks the specific ability to degrade rRNA in intact ribosomes and exhibits an increased unspecific ribonuclease activity and decreased interaction with lipid vesicles. In trying to shed light on these differences, we report here on the three-dimensional structure of the Delta(7-22) alpha-sarcin mutant using NMR methods. We...
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