Article
Tryptophan mutants of intestinal fatty acid-binding protein: ultraviolet absorption and circular dichroism studies.
Archives of biochemistry and biophysics - 15 Nov 2001
Clérico E M, Ermácora M R
Abstract excerpt
An UV absorption and CD study of intestinal fatty acid-binding protein is presented. Since there are only two Trp residues in the molecule, two single-Trp mutants were prepared to deconvolute their signals. The individual contribution of the eight Phe and four Tyr residues was not established; however, Phe global contribution is relatively free of interferences from the other chromophores and was observed...
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