Article
Polymerase chain reaction with additional primers allows identification of amplified DNA and recognition of specific alleles.
Molecular and cellular probes - 1 Feb 1992
Candrian U, Höfelein C, Lüthy J
Abstract excerpt
The use of additional primers in the standard two primer polymerase chain reaction (PCR) is described. This modification allows detection of a target gene in a single reaction, and identification of the amplification product obtained or recognition of a specific allele. The oligonucleotides used are internal to the original amplification primers and amplification-compatible with one of the original primers....
Topics
- Alleles
- Artifacts
- Base Sequence
- DNA, Bacterial
- Escherichia coli
- Gene Amplification
- Listeria monocytogenes
- Molecular Sequence Data
- Oligonucleotide Probes
- Polymerase Chain Reaction