Article
Hemoglobin E detection using PCR with confronting two-pair primers.
Journal of the Medical Association of Thailand = Chotmaihet thangphaet - 1 Nov 2008
Intorasoot Sorasak, Thongpung Rachanu, Tragoolpua Khajornsak, Chottayaporn Mongkol
Abstract excerpt
OBJECTIVES: To develop and apply the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) for detection and identification of hemoglobin E (Hb E). MATERIAL AND METHOD: Fifty unrelated northern Thais were included in the present study. DNA was extracted from peripheral blood mononuclear cells and targeted to amplify by PCR-CTPP. The amplified product was analyzed and compared with the reference...
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