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Article

Membrane topology of the colicin E1 channel using genetically encoded fluorescence.

Biochemistry - 7 Jun 2011

Ho Derek, Lugo Miguel R, Lomize Andrei L, Pogozheva Irina D, Singh Suneel P, Schwan Adrian L, Merrill A Rod

Abstract excerpt

The membrane topology of the colicin E1 channel domain was studied by fluorescence resonance energy transfer (FRET). The FRET involved a genetically encoded fluorescent amino acid (coumarin) as the donor and a selectively labeled cysteine residue tethered with DABMI (4-(dimethylamino)phenylazophenyl-4'-maleimide) as the FRET acceptor. The fluorescent coumarin residue was incorporated into the protein via an...

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Identifiers

PMID: 21528912
DOI: 10.1021/bi101934e

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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