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Article

Mode of action and application of Scorpion primers to mutation detection.

Nucleic acids research - 1 Oct 2000

Thelwell N, Millington S, Solinas A, Booth J, Brown T

Abstract excerpt

Scorpion primers can be used to detect PCR products in homogeneous solution. Their structure promotes a unimolecular probing mechanism. We compare their performance with that of the same probe sequence forced to act in a bimolecular manner. The data suggest that Scorpions indeed probe by a unimolecular mechanism which is faster and more efficient than the bimolecular mechanism. This mechanism is not dependent on...

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Identifiers

PMID: 11000267
DOI: 10.1093/nar/28.19.3752
PMCID: PMC110766

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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