Article
Symmetric snapback primers for scanning and genotyping of the cystic fibrosis transmembrane conductance regulator gene.
Clinical chemistry - 1 Jul 2013
Zhou Luming, Palais Robert A, Ye Felix, Chen Jimmy, Montgomery Jesse L, Wittwer Carl T
Abstract excerpt
BACKGROUND: High-resolution melting of PCR products is an efficient and analytically sensitive method to scan for sequence variation, but detected variants must still be identified. Snapback primer genotyping uses a 5' primer tail complementary to its own extension product to genotype the resulting hairpin via melting. If the 2 methods were combined to analyze the same PCR product, the residual sequencing burden...
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