Article
A method for point mutation analysis that links SSCP and dye primer fluorescent sequencing.
Molecular and cellular probes - 1 Jun 1998
Chiarelli I, Porfirio B, Mattiuz P L, Seri M, Caroli F, Celli I, Romeo G, Volorio S, Zollo M
Abstract excerpt
A method is presented for mutation detection directly from single-strand conformational polymorphism (SSCP) variants. This approach is based on: (i) amplification of the exons to be analysed by SSCP using the forward primer with an eight-base tail to form a universal SSCP cassette; (ii) excision from the gel of the shifted silver-stained bands; (iii) reamplification of the eluted DNAs using, as the forward...
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