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A single-molecule approach to DNA replication in Escherichia coli cells demonstrated that DNA polymerase III is a major determinant of fork speed.

Molecular microbiology - 1 Nov 2013

Pham Tuan Minh, Tan Kang Wei, Sakumura Yuichi, Okumura Katsuzumi, Maki Hisaji, Akiyama Masahiro Tatsumi

Abstract excerpt

The replisome catalyses DNA synthesis at a DNA replication fork. The molecular behaviour of the individual replisomes, and therefore the dynamics of replication fork movements, in growing Escherichia coli cells remains unknown. DNA combing enables a single-molecule approach to measuring the speed of replication fork progression in cells pulse-labelled with thymidine analogues. We constructed a new...

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PMID: 23998701
DOI: 10.1111/mmi.12386

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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