Article
Roles of topoisomerase IV and DNA gyrase in DNA unlinking during replication in Escherichia coli.
Genes & development - 15 Nov 1995
Zechiedrich E L, Cozzarelli N R
Abstract excerpt
For a cell to complete DNA replication, every link between the Watson-Crick strands must be removed by topoisomerases. Previously, we reported that the inhibition of topoisomerase IV (topo IV) leads to the accumulation of catenated plasmid replicons to a steady-state level of approximately 10%. Using pulse labeling with [3H]thymidine in Escherichia coli, we have found that in the absence of topo IV activity,...
Topics
- DNA Replication
- DNA Topoisomerase IV
- DNA Topoisomerases, Type II
- DNA, Bacterial
- Mutation
- Plasmids