Article
Replication arrests during a single round of replication of the Escherichia coli chromosome in the absence of DnaC activity.
Molecular microbiology - 1 Dec 2001
Maisnier-Patin S, Nordström K, Dasgupta S
Abstract excerpt
We used a flow cytometric assay to determine the frequency of replication fork arrests during a round of chromosome replication in Escherichia coli. After synchronized initiation from oriC in a dnaC(Ts) strain, non-permissive conditions were imposed, such that active DnaC was not available during elongation. Under these conditions, about 18% of the cells failed to complete chromosome replication. The sites of...
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