Article
IEM101, a naturally attenuated Vibrio cholerae strain as carrier for genetically detoxified derivatives of cholera toxin.
Vaccine - 15 Aug 2000
Fontana M R, Monaci E, Yanqing L, Guoming Q, Duan G, Rappuoli R, Pizza M
Abstract excerpt
Two mutants of cholera toxin (CTS106 containing a Pro106-->Ser substitution and CTK63 containing a Ser63-->Lys substitution) with greatly reduced or no toxicity respectively, were expressed in the naturally attenuated IEM101 Vibrio cholerae strain (El Tor, Ogawa) which does not express cholera toxin (CT). Expression was driven by the natural promoter of CT, or by a promoter known to induce strong in vivo...
Topics
- Administration, Intranasal
- Animals
- Antibodies, Bacterial
- Cholera
- Cholera Toxin
- Cholera Vaccines
- Disease Models, Animal
- Female
- Mice
- Mice, Inbred C57BL
- Mutation