Article
Characterization and pathogenic significance of Vibrio vulnificus antigens preferentially expressed in septicemic patients.
Infection and immunity - 1 Oct 2003
Kim Young Ran, Lee Shee Eun, Kim Choon Mee, Kim Soo Young, Shin Eun Kyoung, Shin Dong Hyeon, Chung Sun Sik, Choy Hyon E, Progulske-Fox Ann, Hillman Jeffrey D, Handfield Martin, Rhee Joon Haeng
Abstract excerpt
Many important virulence genes of pathogenic bacteria are preferentially expressed in vivo. We used the recently developed in vivo-induced antigen technology (IVIAT) to identify Vibrio vulnificus genes induced in vivo. An expression library of V. vulnificus was screened by colony blot analysis by using pooled convalescent-phase serum that had been thoroughly adsorbed with in vitro-expressed V. vulnificus whole...
Topics
- Animals
- Antigens, Bacterial
- Bacterial Proteins
- Base Sequence
- DNA, Bacterial
- Female
- Gene Expression
- Genes, Bacterial
- HeLa Cells
- Humans
- Male
- Mice
- Middle Aged