[Mismatched PCR combined with restriction fragment length polymorphism assay for identification of hot spot for mutation in core promoter sequence of hepatitis B virus in Chinese isolates].

Abstract excerpt

OBJECTIVES: To investigate the prevalence of the core promoter variant in Chinese HBV carriers and to explore the clinical and epidemiological problems of the variant. METHODS: A novel method for detecting the double mutation in core promoter was developed. By using an antisense primer that starts at nt 1765 and bears a mismatch at nt 1767, we introduced an artificially created Bcl I cleavage site if the double...

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