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Article

Rapid and cost-effective screening of newly identified microsatellite loci by high-resolution melting analysis.

Molecular genetics and genomics : MGG - 1 Oct 2011

Arthofer Wolfgang, Steiner Florian M, Schlick-Steiner Birgit C

Abstract excerpt

This study describes a new method for identifying microsatellite loci that will reliably amplify and show high degree of polymorphism in a given species. Microsatellites are the most powerful codominant markers available today, but the development of novel loci remains a labour-intensive and expensive process. In de novo isolation, approaches using next generation sequencing (NGS) are gradually replacing ones...

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PMID: 21847526
DOI: 10.1007/s00438-011-0641-0

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