Article
Rapid and cost-effective screening of newly identified microsatellite loci by high-resolution melting analysis.
Molecular genetics and genomics : MGG - 1 Oct 2011
Arthofer Wolfgang, Steiner Florian M, Schlick-Steiner Birgit C
Abstract excerpt
This study describes a new method for identifying microsatellite loci that will reliably amplify and show high degree of polymorphism in a given species. Microsatellites are the most powerful codominant markers available today, but the development of novel loci remains a labour-intensive and expensive process. In de novo isolation, approaches using next generation sequencing (NGS) are gradually replacing ones...
Topics
- Animals
- Ants
- Cost-Benefit Analysis
- DNA Primers
- Electrophoresis, Capillary
- Escherichia coli
- Gene Library
- Genetic Techniques
- Genotype
- Microsatellite Repeats
- Nucleic Acid Amplification Techniques
- Nucleic Acid Denaturation
- Polymerase Chain Reaction
- Sequence Analysis, DNA