Article
Approach to genotyping errors caused by nontemplated nucleotide addition by Taq DNA polymerase.
Genome research - 1 Oct 1995
Smith J R, Carpten J D, Brownstein M J, Ghosh S, Magnuson V L, Gilbert D A, Trent J M, Collins F S
Abstract excerpt
Thermostable DNA polymerases can catalyze nontemplated addition of a nucleotide to the 3' end of amplification products. This presents a potential source of error in genotyping studies employing Taq DNA polymerase to amplify microsatellite loci. Although the activity is marker specific, experimental variation is often seen in the degree of modification. Consequently, for a given microsatellite marker, an allele...
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