Article
Microsatellite fragment analysis using the ABI PRISM ® 377 DNA sequencer.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013
Renshaw Mark A, Giresi Melissa, Adams J Orville
Abstract excerpt
The ABI PRISM (®) 377 DNA Sequencer is used for a variety of microsatellite-based research. The platform provides researchers with a cost-effective means for high-throughput genotyping, which can be further optimized by multiplexing microsatellite loci or by using a tail-labeling approach to screen large sets of markers. The goals of this chapter are to present a protocol for performing microsatellite-based...
Topics
- DNA
- Electrophoresis, Polyacrylamide Gel
- Genotype
- Microsatellite Repeats
- Molecular Biology
- Polymerase Chain Reaction
- Sequence Analysis, DNA