Article
Physical association of eukaryotic initiation factor 4G (eIF4G) with eIF4A strongly enhances binding of eIF4G to the internal ribosomal entry site of encephalomyocarditis virus and is required for internal initiation of translation.
Molecular and cellular biology - 1 Aug 2000
Lomakin I B, Hellen C U, Pestova T V
Abstract excerpt
Mammalian eukaryotic initiation factor 4GI (eIF4GI) may be divided into three similarly sized regions. The central region (amino acids [aa] 613 to 1090) binds eIF3, eIF4A, and the encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES) and mediates initiation on this RNA. We identified the regions of eIF4GI that are responsible for its specific interaction with the IRES and that are required to...
Topics
- Animals
- Binding Sites
- Encephalomyocarditis virus
- Eukaryotic Initiation Factor-4A
- Eukaryotic Initiation Factor-4G
- Mutation
- Peptide Initiation Factors
- Protein Binding
- Protein Biosynthesis
- Ribosomal Proteins