Article
Arrayed primer extension reaction for genotyping on oligonucleotide microarray.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008
Pullat Janne, Metspalu Andres
Abstract excerpt
Arrayed primer extension reaction (APEX) is a straightforward and robust enzymatic genotyping method in which hundreds to thousands of variations in the genome are simultaneously analyzed in a single multiplexed reaction. It differs from allele-specific hybridization in that the genotype information in APEX is obtained by single base extension, performed by a specific DNA polymerase, together with four different...
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