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Article

SNP typing by apyrase-mediated allele-specific primer extension on DNA microarrays.

Nucleic acids research - 1 Aug 2002

O'Meara Deirdre, Ahmadian Afshin, Odeberg Jacob, Lundeberg Joakim

Abstract excerpt

This study reports the development of a microarray-based allele-specific extension method for typing of single nucleotide polymorphisms (SNPs). The use of allele-specific primers has been employed previously to identify single base variations but it is acknowledged that certain mismatches are not refractory to extension. Here we have overcome this limitation by introducing apyrase, a nucleotide-degrading enzyme,...

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Identifiers

PMID: 12140337
DOI: 10.1093/nar/gnf074
PMCID: PMC137093

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Source receipt: pubmed, retrieved 7 Sept 2026.

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