[Studies of gene regulation of de novo biosynthetic pathway of purine in Salmonella typhimurium. X. Isolation of purR(am) mutants and preliminary studies of amino acid substitution].

Abstract excerpt

Starting from a super-repressed mutant of purR, 3-18, 439 independent candidates of purR- mutants were isolated by using NCE selecting plate with lactose as sole carbon source. Among these mutants. 11 amber mutants were detected by introducing a tRNA suppressor gene. Cotransduction analysis proved that the amber mutation sites of 11 amber mutants all located on purR. Amino acid substitution experiments were...

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