Article
A mutant of Sindbis virus that is resistant to pyrazofurin encodes an altered RNA polymerase.
Virology - 20 Jun 2000
Lin Y H, Yadav P, Ravatn R, Stollar V
Abstract excerpt
Pyrazofurin (PZF), a cytidine analog and an inhibitor of orotate monophosphate decarboxylase, has been shown to decrease the levels of UTP and CTP in treated cells. When Sindbis virus (SV)-infected Aedes albopictus cells were treated with PZF, the yield of virus was reduced 100- to 1000-fold. By serial passage of our standard SV(STD) in Ae. albopictus cells in the presence of increasing concentrations of PZF, a...
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