Article
Mutations which alter the level or structure of nsP4 can affect the efficiency of Sindbis virus replication in a host-dependent manner.
Journal of virology - 1 Jun 1990
Lemm J A, Durbin R K, Stollar V, Rice C M
Abstract excerpt
Two mutants of Sindbis virus have been isolated which grow inefficiently at 34.5 degrees C in mosquito cells yet replicate normally in chicken embryo fibroblast cells at the same temperature. In addition, these mutants exhibit temperature-sensitive growth in both cell types and are RNA- at the no...
Topics
- Aedes
- Alphavirus
- Amino Acid Sequence
- Animals
- Base Sequence
- Capsid
- Cell Line
- Cells, Cultured
- Chick Embryo
- Cloning, Molecular
- Codon
- DNA, Viral
- Genes, Viral
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Phenotype