Restriction of a Sindbis virus mutant in BHK cells and relief of the restriction by the addition of adenosine.

Abstract excerpt

SV(PZF) is a mutant of Sindbis virus (SV) which we selected on the basis of its ability to replicate in mosquito cells treated with pyrazofurin (PZF), a drug which inhibits pyrimidine nucleotide biosynthesis (Lin et al., 2000, Virology 272, 61-71). Three mutations, A6627U, A7543U, and C7593A, were identified in the nsP4 (the viral RNA polymerase) coding region, which were required for the PZF-resistant phenotype....

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Adenosine
• Share to Cell Line
• Share to Cricetinae