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Article

Effects on interaction kinetics of mutations at the VH-VL interface of Fabs depend on the structural context.

Journal of molecular recognition : JMR - 1 Jan 2000

Khalifa M B, Weidenhaupt M, Choulier L, Chatellier J, Rauffer-Bruyère N, Altschuh D, Vernet T

Abstract excerpt

The influence of framework residues belonging to VH and VL modules of antibody molecules on antigen binding remains poorly understood. To investigate the functional role of such residues, we have performed semi-conservative amino acid replacements at the VH-VL interface. This work was carried out with (i) variants of the same antibody and (ii) with antibodies of different specificities (Fab fragments 145P and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 10867708
DOI: 10.1002/1099-1352(200005/06)13:3<127::AID-JMR495>3.0.CO;2-9

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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